The three dimensional ‘nucleome’
The nucleus is the information processing center of the cell. Signals are received from the cell surface or the cytosol and interpreted into a transcriptional response. Similarly, processes like DNA replication and DNA repair are coordinated within the nucleus. In recent years, the combined use of novel imaging tools, structural analyses, cell biological and genomic studies have led to major progresses in our understanding on how these reactions are coordinated in time and space within the nucleus to form the three dimensional ‘nucleome’. Lectures and class discussions will use the primary literature to look at these cell biological aspects of molecular biology, to offer perspectives on future developments and highlight the existing conceptual and technical problems. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish some major findings in nuclear cell biology. Some examples of topics include genome and systems biology, nuclear organization and chromatin structure, nuclear bodies, RNA dynamics and distribution and X inactivation.
Recent studies in the field of nuclear architecture will be reviewed and the impact on key processes such as transcription regulation, replication and DNA repair will be emphasized. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish major findings in nuclear cell biology. Some examples of topics include genome and systems biology, nuclear organization and chromatin structure, nuclear bodies, RNA dynamics and distribution and X inactivation.
The nucleus is the information processing center of the cell. Signals are received from the cell surface or the cytosol and interpreted into a transcriptional response. Similarly, processes like DNA replication and DNA repair are coordinated within the nucleus. In recent years, the combined use of novel imaging tools, structural analyses, cell biological and genomic studies have led to major progresses in our understanding on how these reactions are coordinated in time and space within the nucleus to form the three dimensional ‘nucleome’. Lectures and class discussions will use the primary literature to look at these cell biological aspects of molecular biology, to offer perspectives on future developments and highlight the existing conceptual and technical problems. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish some major findings in nuclear cell biology. Some examples of topics include genome and systems biology, nuclear organization and chromatin structure, nuclear bodies, RNA dynamics and distribution and X inactivation.
Recent studies in the field of nuclear architecture will be reviewed and the impact on key processes such as transcription regulation, replication and DNA repair will be emphasized. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish major findings in nuclear cell biology. Some examples of topics include genome and systems biology, nuclear organization and chromatin structure, nuclear bodies, RNA dynamics and distribution and X inactivation.
- Teacher: שרו אליצור-שלזינגר