This course aims to provide basic knowledge about cellular release systems )extracellular vesicles including exosomes(, and their macromolecules cargos: mRNA, microRNA(miRNA), and other non-coding RNAs
EV history, nomenclature, biogenesis, We will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs.
The release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques.
In this course, we will focus on one of the cargos of the EVs: the miRNAs.
biogenesis and regulation of miRNAs, their post-transcription regulation of gene expression. The course will introduce students to bioinformatic tools and databases for the analysis of miRNAs.
EV history, nomenclature, biogenesis, We will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs.
The release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques.
In this course, we will focus on one of the cargos of the EVs: the miRNAs.
biogenesis and regulation of miRNAs, their post-transcription regulation of gene expression. The course will introduce students to bioinformatic tools and databases for the analysis of miRNAs.
- Teacher: Regina Gerstl